human lung squamous carcinoma cell lines h226 (ATCC)
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human lung squamous carcinoma cell lines h226
Human Lung Squamous Carcinoma Cell Lines H226, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human lung squamous carcinoma cell lines h226/product/ATCC
Average 96 stars, based on 927 article reviews
Human Lung Squamous Carcinoma Cell Lines H226, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human lung squamous carcinoma cell lines h226/product/ATCC
Average 96 stars, based on 927 article reviews
human lung squamous carcinoma cell lines h226 - by Bioz Stars,
2026-03
96/100 stars
Images
1) Product Images from "Hesperetin induces apoptosis in lung squamous carcinoma cells via G 2 /M cycle arrest, inhibition of the Notch1 pathway and activation of endoplasmic reticulum stress"
Article Title: Hesperetin induces apoptosis in lung squamous carcinoma cells via G 2 /M cycle arrest, inhibition of the Notch1 pathway and activation of endoplasmic reticulum stress
Journal: International Journal of Molecular Medicine
doi: 10.3892/ijmm.2025.5518
Figure Legend Snippet: Effect of HST on the survival rate of H226 and H1703 cells.
Techniques Used:
Figure Legend Snippet: HST induces G 2 /M phase arrest in lung squamous cell carcinoma cells. (A) Flow cytometry was used to assess the cell cycle distribution of H226 and H1703 cells after 48 h of treatment with various concentrations of HST. (B) Western blotting was used to evaluate the expression levels of cell cycle-related proteins cyclin B1 and CDK1 in H226 and H1703 cells after 48 h of treatment with different concentrations of HST. ns P>0.05, * P<0.05, ** P<0.01, *** P<0.001 vs. control group. HST, hesperetin.
Techniques Used: Flow Cytometry, Western Blot, Expressing, Control
Figure Legend Snippet: HST induces apoptosis in lung squamous cell carcinoma cells. (A) Annexin V-FITC/PI staining combined with flow cytometry was used to determine the apoptosis rates of H226 and H1703 cells after 48 h of intervention with different HST concentrations. (B) JC-1 staining and flow cytometry determined the mitochondrial membrane potential levels of H226 and H1703 cells after 48 h of intervention with different HST concentrations. (C) Western blotting was used to measure the expression levels of the apoptosis-related proteins Bax, Bcl-2, caspase-3 and cleaved caspase-3 in H226 and H1703 cells after 48 h of intervention with HST at given concentrations. ns P>0.05, * P<0.05, ** P<0.01, *** P<0.001 vs. control group. HST, hesperetin.
Techniques Used: Staining, Flow Cytometry, Membrane, Western Blot, Expressing, Control
Figure Legend Snippet: HST induces the apoptosis of lung squamous cell carcinoma cells by activating the endoplasmic reticulum stress pathway. (A) Western blotting detected the expression levels of endoplasmic reticulum stress-related proteins Grp78, P-eIF2α, eIF2α and CHOP in H226 and H1703 cells treated with different concentrations of HST for 48 h. (B) H1703 cells were pretreated with 500 μ M 4-PBA for 6 h and were treated with 150 μ M HST for 48 h. The protein expression levels of Grp78, P-eIF2α, eIF2α, Bax, caspase-3 and cleaved caspase-3 were detected by western blotting. ns P>0.05, * P<0.05, ** P<0.01, *** P<0.001 vs. control group; # P<0.05, ## P<0.01, ### P<0.001 vs. 150 μ M HST group. 4-PBA, 4-phenylbutyric acid; Grp78, glucose-regulated protein 78; HST, hesperetin; P-, phosphorylated.
Techniques Used: Western Blot, Expressing, Control
Figure Legend Snippet: HST induces the apoptosis of lung squamous cell carcinoma cells by inhibiting the Notch1 signaling pathway. (A) Western blotting was used to detect the expression levels of the Notch1 signaling pathway proteins Notch1 and Hes-1 in H226 and H1703 cells treated with different concentrations of HST for 48 h. (B) H1703 cells were pretreated with 4 μ M Jagged-1 for 8 h, then treated with 150 μ M HST for 48 h. The protein expression levels of Notch1, Hes-1, Bax, caspase-3 and cleaved caspase-3 were detected by western blotting. ns P>0.05, * P<0.05, ** P<0.01, *** P<0.001 vs. control group; # P<0.05, ## P<0.01, ### P<0.001 vs. 150 μ M HST group. HST, hesperetin.
Techniques Used: Western Blot, Expressing, Control
Figure Legend Snippet: HST inhibits the growth of H226 nude mouse transplanted tumors in vivo . (A) Volume of transplanted tumors in nude mice. (B) Weight of the transplanted tumors in nude mice was recorded, and the inhibition rate of tumor weight was calculated. (C) Morphological characteristics of transplanted tumor tissues in nude mice were documented. Scale bar, 300 μ m. (D) Body weight of nude mice was determined. (E) Histopathological features of key organs (heart, liver and kidney) were determined. Scale bar, 300 μ m. (F) Western blotting was used to detect the expression levels of Notch1, Grp78, eIF2α and P-eIF2α in transplanted tumor tissues of the nude mice. * P<0.05, ** P<0.01, *** P<0.001 vs. control group. Grp78, glucose-regulated protein 78; HST, hesperetin; P-, phosphorylated.
Techniques Used: In Vivo, Inhibition, Western Blot, Expressing, Control